2024 STSM Experience
Scientific Part;
Embarking on a Short-Term Scientific Mission (STSM) as part of the COST project was an enlightening journey into the realm of plastic additives and nanomaterials, particularly focusing on their ecological effects. This initiative, aimed at bridging gaps in microplastics and nanoplastics research, provided me with a unique platform to explore the impacts of phthalates and nanomaterials on environmental systems, specifically soil organisms and zebrafish embryos. My journey began with a focus on nanoplastics, but an intriguing shift allowed me to delve into the effects of phthalates, a pivot that enriched my research experience.
Phthalates, such as Diisononyl phthalate (DINP) and Dioctyl terephthalate (DOTP), are ubiquitous in consumer products, enhancing flexibility but often leaching into ecosystems, posing significant risks. Despite their prevalent use, there is limited research on their long-term ecological impacts, particularly on soil organisms like collembolans, Folsomia candida. This gap underscores the necessity of our investigation, aiming to inform future regulations and ecological safety standards.
Upon joining the host institution, I was introduced to the laboratory under the guidance of hosts. They facilitated my integration into the research environment and outlined the experimental designs, starting with an avoidance test for DINP. This test aimed to assess how various concentrations of DINP impacted the behavior of Folsomia candida, following ISO 17512-2 guidelines. The process involved preparing soil samples with varying DINP concentrations, ensuring complete solvent evaporation, and introducing organisms into these environments to observe their distribution after 48 hours.
Concurrently, I initiated a reproduction test to evaluate the long-term effects of DINP on Folsomia candida, adhering to OECD guidelines (test number 232). This involved preparing spiked soils, introducing age-synchronized individuals, and monitoring their survival and reproduction over a four-week period, with weekly feeding and watering. Throughout this process, I honed my skills in statistical analysis and image processing using ImageJ, which will be pivotal in determining the impact of phthalates on organism survival and reproduction.
Beyond phthalates, I also participated in a nanomaterials experiment focusing on zebrafish embryos. This exposure broadened my experimental repertoire, involving the preparation of nanomaterial solutions, maintaining optimal conditions for embryos, and monitoring their development over 96 hours. Such multidisciplinary experiences have significantly contributed to my understanding of the potential environmental risks associated with phthalates and nanomaterials.
The achievements of this STSM are manifold. We successfully assessed the behavioral responses of Folsomia candida to DINP, providing key insights into the short-term effects of this plastic additive. Future tests involving DOTP are planned, with my continued involvement in data analysis ensuring the continuity of this research. The reproduction test, which examines the long-term effects of DINP, is ongoing, with remote collaboration facilitating my engagement in data analysis and interpretation.
In conclusion, this STSM has been a rewarding experience, aligning with the Action's objectives and contributing valuable insights to plastics research. The training provided by my hosts has equipped me with skills applicable to my future research, fostering a partnership that promises further advancements in understanding and mitigating the ecological impacts of plastic additives and nanomaterials. This experience has not only solidified my research capabilities but also opened doors for future collaborations that will benefit both the scientific community and public environmental health.
Social Part;
The journey began in earnest after my application was approved in May 2024. We scheduled the STSM to start in October, perfectly fitting into my chaotic life as I juggled a second master’s program. Little did I know that this timing would add a layer of complexity to my adventure.
The travel day was a test of endurance. After a grueling 23-hour journey with connecting flights and a frantic rush through Lisbon’s passport control, I finally arrived at my hostel. Exhausted and starving, I dropped my bags and ventured out for a late-night meal. The streets of Lisbon were alive with energy, a warm welcome to this vibrant city. Despite my exhaustion, I couldn’t resist wandering the lively streets, soaking in the happy atmosphere.
The following morning, I explored Lisbon with fresh eyes. From iconic tramway lines to the bustling streets filled with history, I was captivated. A city tour led by a charming French guide enriched my understanding of Lisbon, and a visit to the Calouste Gulbenkian Museum was the perfect cultural immersion before heading to Aveiro.
Aveiro, with its unexpected charm, was larger than I had imagined. My first night there was a comedy of errors, stepping in dog poo and navigating a midnight walk to find food. Yet, even these small mishaps were part of the adventure. The next day, meeting my host at the lab was a turning point. The lab was a haven of organized chaos, and I immediately felt at home. The lively campus and the bustling lab environment were invigorating.
One of the highlights of my journey was a trip to Costa Nova. Facing the Atlantic Ocean for the first time was a humbling experience. The vastness and power of the ocean were awe-inspiring, a reminder of nature’s grandeur. The colorful houses of Costa Nova added a whimsical touch to this memorable day.
Back in the lab, I was immersed in experiments, shuttling between avoidance tests and zebrafish studies. The process was intricate and demanding, but the camaraderie with my hosts made it enjoyable. Lunchtimes were filled with laughter and valuable cultural exchanges, with plenty of recommendations for places to visit.
Exploring Coimbra on a day trip was another highlight. The city’s rich history and architecture were a delightful surprise, and I found myself lost in its charming streets. Meanwhile, the vibrant library back in Aveiro became my second home, a perfect spot for catching up on math and reflecting on my experiences.
As my time in Portugal drew to a close, I felt a sense of accomplishment and gratitude. The people I met, the experiences I had, and the knowledge I gained were invaluable. My hosts were not just colleagues but kindred spirits, sharing a passion for science and a commitment to doing good in the world. It was a journey of discovery, not just of nanoplastics and phthalates, but of myself and the incredible people I met along the way.
Leaving Portugal, I am filled with hope for future collaborations and a deep appreciation for the warmth and hospitality I experienced. This STSM was more than a scientific mission; it was a personal journey that enriched my life in countless ways. As I head back home, I carry with me fond memories and a renewed sense of purpose, eager to see where the journey takes me next.